mouse anti human antibody il 6  (InvivoGen)

Journal: Microbiology Spectrum

Article Title: Bacterial Lipoproteins Shift Cellular Metabolism to Glycolysis in Macrophages Causing Bone Erosion

doi: 10.1128/spectrum.04293-22

Figure Lengend Snippet: P2C induces significantly higher cytokine production than P3C in BMDM and MM6 cells. Cytokine production of BMDM (A, B) and BMM (C, D) stimulated with 100 ng/mL of indicated LPP analogues or LPS was assessed as described in Materials and Methods. Control samples (unsti.) were used without adding any stimulators. mTNF-α and mIL6 were measured by ELISA from supernatant of 20 h stimulation. The data were obtained from cells isolated from 3 to 6 mice. Error bars indicate mean ± SEM. Statistical significances were calculated between the treated cells compared to control (non) by one-way ANOVA using Tukey’s multiple-comparison test: *, P < 0.05; **, P < 0.01; ***, P < 0.001. (E to G) Cytokine production of MM6 stimulated with 100 ng/mL of indicated LPP analogues or LPS was assessed as described in Materials and Methods. hTNF-α values were measured by ELISA from supernatant of 5 h stimulation, and hIL6 and hIL-10 were measured by ELISA from supernatant of 18 h stimulation. Three independent experiments were carried out in triplicate. Error bars indicate SEM. Statistical significances were calculated between the treated cells and control (unsti.) by one-way ANOVA using Tukey’s multiple-comparison test; *, P ≤ 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: For the blocking assay in MM6 cells, 5 × 10 5 cells were incubated for 1 h with either 1 μg/mL of rabbit anti-human antibodies TNF (catalog no. D1B4; Cell Signaling, Leiden, Netherlands), 1 μg/mL of mouse anti-human antibody IL-6 (catalog no. mabg-hil6-3; InvivoGen, Toulouse, France), 1 μg/mL of rat antihuman antibody IL-10 (catalog no. AHC0103; Thermo Fisher, Schwerte, Germany), or 1 μg/mL mouse anti-human monoclonal antibody IgG1 (catalog no. bgal-mab1-ctrlm; InvivoGen, Toulouse, France) as isotype control.

Techniques: Analogues, Control, Enzyme-linked Immunosorbent Assay, Isolation, Comparison

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Concomitant NAFLD Facilitates Liver Metastases and PD-1-Refractory by Recruiting MDSCs via CXCL5/CXCR2 in Colorectal Cancer

doi: 10.1016/j.jcmgh.2024.04.008

Figure Lengend Snippet: Immunohistochemical Staining

Article Snippet: Rabbit anti-mouse/human IL6 , N/A , Proteintech; 21865-1-AP.

Techniques: Immunohistochemical staining

Journal: PLOS ONE

Article Title: Crystalline silica-exposed human lung epithelial cells presented enhanced anchorage-independent growth with upregulated expression of BRD4 and EZH2 in autocrine and paracrine manners

doi: 10.1371/journal.pone.0285354

Figure Lengend Snippet: (A) ELISA of EGF, TNF-α, IL-6, and TGF-β1 was performed with the culture supernatants of nonadherent NL20, BEAS-2B, and 16HBE 4–6 days after incubation with autocrine CS or BPDE CM compared with the autocrine control CM. ELISA of EGF, TNF-α, and IL-6 was also conducted with the culture supernatants 48 h after exposure to CS in adherent bronchial cell lines. Asterisks indicate a significant difference in concentration (*p < 0.05, **p < 0.01). Relative values are shown as mean ± SD from three independent experiments. (B) Anchorage-independent growth of NL20, BEAS-2B, and 16HBE cells was measured using MTT 5–6 days after the administration of recombinant human EGF, TNF-α, and IL-6. The MTT assay was performed for autocrine CS CM-incubated nonadherent NL20 and BEAS-2B cells four and five days after EGF-neutralizing antibody (NtAb) treatment, respectively, and for autocrine CS CM-incubated nonadherent 16HBE cells, five days after TNF-α NtAb treatment. The MTT assay was also performed for autocrine CS CM-incubated nonadherent bronchial cell lines five days after IL-6 NtAb administration. Asterisks indicate a significant difference in growth (*p < 0.05, **p < 0.01). Relative values are shown as mean ± SD from three independent experiments. (C) Expression of BRD4 and EZH2 in nonadherent 16HBE cells three days after the administration of recombinant human TNF-α determined using western blotting and the expression of BRD4 and EZH2 3–4 days after treatment of nonadherent NL20 and BEAS-2B cells with recombinant human EGF.

Article Snippet: Mouse anti-human EGF neutralizing antibody (NtAb), goat anti-human TNF-α NtAb, and mouse anti-human IL-6 NtAb were obtained from R&D Systems.

Techniques: Enzyme-linked Immunosorbent Assay, Incubation, Control, Concentration Assay, Recombinant, MTT Assay, Expressing, Western Blot